We use essential cookies to operate our site. With your consent, we may also use non-essential cookies to improve your user experience and to analyze website traffic. Click "Accept" to accept cookies and go directly to the site, click "Reject" to reject all but cookies strictly necessary for the functioning of this site. You can reset your cookie settings at any time by visiting your Bioz "my account" page and selecting the "reset cookie preferences" link.

Accept Reject

Overview
Images
Article Snippets
home > search results > product details
star_border
     Loading Product Details ...      Welcome to Your Next Discovery   
93 / 100
Bioz Stars

Santa Cruz  
Open an account
already a user? Sign in
are you a supplier? Contact us
Product Images (6) - All
All
Activation Assay
Expressing
Immunostaining
Labeling
MANN-WHITNEY
Mutagenesis
Staining
Suprachiasmatic Function in a Circadian Period Mutant: Duper alters light-induced activation of Vasoactive Intestinal Peptide cells and PERIOD1 immunostaining 
star_border
Light exposure in early subjective night induced c-FOS-ir but not <t>PER1-ir</t> at the 2h survival point. (A) c-FOS-ir was not detected in the SCN of control hamsters 2h after cage movement, but after a 15’ light pulse at CT15, c-FOS-ir cells were found in both ventral and dorsal mid-SCN in similar numbers and intensities in wild type and duper mutant hamsters. (B) PER1 immunoreactivity in the SCN as a whole was similar in wild type and duper mutant hamsters. Neither the number of PER1-ir cells (C) nor PER1 staining intensity (mean±SEM, arbitrary units; D) was affected by the light pulse at this time point, although the light pulse resulted in a more ventral distribution of PER1-ir cells in duper hamsters [n.d. = not detectable; n.t. = not tested; Mann-Whitney test, *p=0.015].
Suprachiasmatic Function in a Circadian Period Mutant: Duper alters light-induced activation of Vasoactive Intestinal Peptide cells and PERIOD1 immunostaining 
star_border
Effects of the duper mutation upon activation depends upon cell type. (A) A light pulse at CT15 increased the number of cFOS-ir/VIP-ir co-labeled cells 2h later in both duper (p=0.008) and WT (p=0.04) hamsters. The inductive effect of light on this population was greater in duper than WT hamsters (**, p= 0.02). (B) cFOS-ir intensity in VIP-ir cells increased in duper but not WT hamsters 2h after a light pulse at CT15 (*, p=0.05). (C) Light pulse at CT15 did not influence the percentage of VIP-ir cells containing <t>PER1</t> 2h later in either WT (p=0.81) or duper (p=0.57) hamsters. (D). Intensity of PER1-ir staining in VIP cells was not affected by light exposure or genotype.
Suprachiasmatic Function in a Circadian Period Mutant: Duper alters light-induced activation of Vasoactive Intestinal Peptide cells and PERIOD1 immunostaining 
star_border
Photomicrographs illustrating immunostaining for cFOS-VIP, <t>PER1-VIP,</t> and AVP-cFOS-PER1. (A-D) Representative images of a double labeled SCN in Experiment 1. cFOS-ir (red) and VIP-ir (green) are shown in WT (A, B) and duper (C, D) hamsters 2h after either a cage movement (control procedure, A, C) or a 15-min light pulse (B, D) at CT15 (40X). (E-J) Double labeled SCN in Experiment 2. PER1-ir (red) and VIP-ir (green) are detected in WT (E, F) and duper (G, H) hamsters 6h after either a cage movement (E, G) or a 15-min light pulse (F, H) at CT15 (20X). White arrows indicate VIP co-labeled cells; light blue arrows indicate cells stained for VIP only. (I, J) Representative 10X images of WT SCN 1h after a light pulse at CT15 triple labeled (I) for AVP (blue), cFOS (green) and VIP (red) or double labeled (J) for PER1 and VIP. Note that PER1-ir cells are medial to cFOS-ir cells.
Suprachiasmatic Function in a Circadian Period Mutant: Duper alters light-induced activation of Vasoactive Intestinal Peptide cells and PERIOD1 immunostaining The European journal of neuroscience, 2019 Dec 01
"Light exposure in early subjective night induced c-FOS-ir but not <t>PER1-ir</t> at the 2h survival point. (A) c-FOS-ir was not detected in the SCN of control hamsters 2h after cage movement, but after a 15’ light pulse at CT15, c-FOS-ir cells were found in both ventral and dorsal mid-SCN in similar numbers and intensities in wild type and duper mutant hamsters. (B) PER1 immunoreactivity in the SCN as a whole was similar in wild type and duper mutant hamsters. Neither the number of PER1-ir cells (C) nor PER1 staining intensity (mean±SEM, arbitrary units; D) was affected by the light pulse at this time point, although the light pulse resulted in a more ventral distribution of PER1-ir cells in duper hamsters [n.d. = not detectable; n.t. = not tested; Mann-Whitney test, *p=0.015]. "
Protocol Conditions
Article Snippets for All Techniques